ZEISS Lattice SIM 5​

SIM² is a groundbreaking image reconstruction algorithm that increases the resolution and sectioning quality of structured illumination microscopy data. SIM² is compatible with all SIM imaging modes and fully integrated in ZEISS ZEN software.​

​Unlike conventional reconstruction algorithms, SIM² is a two-step image reconstruction algorithm. First, order combination, denoising, and frequency suppression filtering are performed. All the effects resulting from these digital image manipulations are translated into a digital SIM point spread function (PSF). The subsequent iterative deconvolution uses this PSF. Similar to the advantages of using experimental PSF for deconvolution of hardware-based microscopy data, the SIM² algorithm is superior to conventional one-step image reconstruction methods in terms of resolution, sectioning, and robustness.​

Burst mode processing uses the rolling window approach to let you observe processes in your living samples at up to 255 fps. Since Burst mode is a post-acquisition step, you have the flexibility to use it with previously acquired data sets. You decide how much temporal resolution is required for your data analysis.​

For demanding fast imaging in 3D, the Leap mode acquisition enables you to reduce your imaging time and lower the light exposure on your sample. This works by imaging only every third plane, for three-times higher volume imaging speed and three-times fewer light exposures. ​

The Lattice SIM illumination pattern exhibits both higher contrast and deeper sample penetration as compared to classical SIM. Achieve super-resolution images along with high-quality sectioning even in thick or scattering samples.​

​A novel clearing and embedding technology developed by Prof. Tang and his team (Hsiao et al., Nature Communications 2023) combined with the robust Lattice SIM illumination pattern and excellent image reconstruction technology enabled imaging throughout an entire mouse intestine section of ~200 µm thickness. Networks of blood vessels and nerves can be visualized with finest details even at this depth.​

ZEISS Lattice SIM 5 combines high speed imaging with incredible light efficiency, low photon dosage and sensitivity. You can observe cellular, subcellular, and even sub-organelle structures in living specimens in 2D and 3D over time. ​

​Mitochondria are highly dynamic organelles that constantly undergo fusion and fission events to ensure proper distribution of ATP across the cell. In order to do their job, they are known to interact with many other subcellular compartments including microtubules, which they ride on to get to their destinations, or the ER, which wraps around mitochondria to initially constrict their diameter before fission events.​